DNA

Part:BBa_K5096070

Designed by: Seonwoo Im   Group: iGEM24_Lambert-GA   (2024-09-30)

sg70 This sequence codes for the guide sequence of the sgRNA70. This sequence is derived from the inhA gene of Mycobacterium tuberculosis. Among M. tuberculosis genes, the inhA gene was selected due to its low mutation rate and its critical role in M. tuberculosis’s pathogenicity and survival (Marrakchi et al., 2014). By inhibiting inhA with our CRISPRi system, the sgRNA70 aims to disrupt M. tuberculosis's pathogenicity. This part is critical for locating the non-coding strand of the target DNA sequence, since it has complementary base pairs of inhA target.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None